Our Technology

Our technology is unique in its ability to recover populations of specific cells based upon their biophysical properties in a sterile environment and without impacting the cells, enabling their maintenance and viability for further analysis and use in biomedical research.. As the technology does not use cell markers, cells are recovered in their native, unbiased state, and previously undetected rare cell populations can be recovered – a powerful tool for advancing our new understanding of the tumor microenvironment.

Our technology recognizes that all cells have a unique biophysical fingerprint. Our dielectrophoretic field technology separates cells with the same phenotype, allowing for the study of individual cells within a homogeneous cell population. Cell separation is rapid, and  our proprietary microfluidic devices provide a sterile environment for cell recovery.

Because CytoRecovery’s cell sorting is marker independent, even cells without known protein markers can be recovered. Where standard cell recovery technology may require at least 1 million cells to find a subpopulation, cDEP allows for the recovery of single cells. Cells remain viable, allowing researchers to conduct analysis on the cell subpopulations that have been recovered.

Previous Publications

Selected publications utilizing CytoRecovery technology from our inventors and scientific advisory team.

  • Hyler AR, Hong D, Davalos RV, Swami NS, Schmelz EM. A novel ultralow conductivity electromanipulation buffer improves cell viability and enhances dielectrophoretic consistency. Electrophoresis. 2021; https://doi.org/10.1002/elps.202000324
  • Farmehini V, Varhue W, Salahi A, Hyler AR, Cemazar J, Davalos RV, et al. On-Chip Impedance for Quantifying Parasitic Voltages During AC Electrokinetic Trapping. Ieee Transactions Bio-medical Eng. 2019;67: 1664–1671. doi:10.1109/tbme.2019.2942572
  • Salahi A, Varhue WB, Farmehini V, Hyler AR, Schmelz EM, Davalos RV, et al. Self-aligned microfluidic contactless dielectrophoresis device fabricated by single-layer imprinting on cyclic olefin copolymer. Anal Bioanal Chem. 2020;412: 3881–3889. doi:10.1007/s00216-020-02667-9
  • Farmehini V, Rohani A, Su Y-H, Swami NS. A wide-bandwidth power amplifier for frequency-selective insulator-based dielectrophoresis. Lab Chip. 2014;14: 4183–7. doi:10.1039/c4lc00801d
  • Douglas TA, Alinezhadbalalami N, Balani N, Schmelz EM, Davalos RV. Separation of Macrophages and Fibroblasts Using Contactless Dielectrophoresis and a Novel ImageJ Macro. Bioelectr. 2019;1: 49–55. doi:10.1089/bioe.2018.0004
  • Douglas TA, Cemazar J, Balani N, Sweeney DC, Schmelz EM, Davalos RV. A feasibility study for enrichment of highly aggressive cancer subpopulations by their biophysical properties via dielectrophoresis enhanced with synergistic fluid flow. Electrophoresis. 2017;38: 1507–1514. doi:10.1002/elps.201600530
  • Cemazar J, Douglas TA, Schmelz EM, Davalos RV. Enhanced contactless dielectrophoresis enrichment and isolation platform via cell-scale microstructures. Biomicrofluidics. 2016;10: 14109. doi:10.1063/1.4939947
  • Salmanzadeh A, Sano MB, Gallo-Villanueva RC, Roberts PC, Schmelz EM, Davalos RV. Investigating dielectric properties of different stages of syngeneic murine ovarian cancer cells. Biomicrofluidics. 2013;7: 011809. doi:10.1063/1.4788921
  • Salmanzadeh A, Kittur H, Sano MB, Roberts PC, Schmelz EM, Davalos RV. Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis. Biomicrofluidics. 2012;6: 24104–2410413. doi:10.1063/1.3699973
  • Salmanzadeh A, Romero L, Shafiee H, Gallo-Villanueva RC, Stremler MA, Cramer SD, et al. Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature. Lab Chip. 2012;12: 182–9. doi:10.1039/c1lc20701f
  • Salmanzadeh A, Elvington ES, Roberts PC, Schmelz EM, Davalos RV. Sphingolipid metabolites modulate dielectric characteristics of cells in a mouse ovarian cancer progression model. Integr Biology Quantitative Biosci Nano Macro. 2013;5: 843–852. doi:10.1039/c3ib00008g
  • Sano MB, Salmanzadeh A, Davalos RV. Multilayer contactless dielectrophoresis: theoretical considerations. Electrophoresis. 2012;33: 1938–46. doi:10.1002/elps.201100677
  • Shafiee H, Sano MB, Henslee EA, Caldwell JL, Davalos RV. Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP). Lab Chip. 2010;10: 438. doi:10.1039/b920590j
  • Shafiee H, Caldwell JL, Sano MB, Davalos RV. Contactless dielectrophoresis: a new technique for cell manipulation. Biomed Microdevices. 2009;11: 997–1006. doi:10.1007/s10544-009-9317-5

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